Chromatography
GAS CHROMATOGRAPHY
COUPLED TO MASS SPECTROMETRY
It allows the separation and identification of complex mixtures.
The mass spectrometer can operate in Full Scan mode (complete scan) or in SIM mode (observation of a previously selected ion or group of ions).
LIQUID CHROMATOGRAPHY
WITH FLUORESCENCE DETECTOR (HPLC-FL).
For compounds that exhibit natural fluorescence, as well as those that can be converted into fluorescent compounds.
- ION CHROMATOGRAPH WITH AMPEROMETRIC DETECTOR AND CONDUCTIVITY DETECTOR.
It is a variant of High Performance Liquid Chromatography (HPLC). It is an effective method for the separation and determination of ions, based on the use of ion exchange resins.
Amperometric detector: measures the intensity generated when applying a potential sweep.
Conductivity detector: measures the conductance of the analyte ion when it passes through the conductivity cell.
Instrumental Facilities
GAS CHROMATOGRAPHY
COUPLED TU MASS SPECTROMETRY:
Finnigan Trace GC Ultra Gas Chromatograph,
Finnigan Trace DSQ Mass Spectrometry Detector
, Electron Impact Ionization Source,
AI 3000 Automatic Sampler, Split/Splitless Injector
, Xcalibur Logical Work Support,
Purge and Trap System: Velocity XPT, Teedyne Tekmar
LIQUID CHROMATOGRAPHY
Thermo liquid chromatograph with fluorescence detector (HPLC-FL), model Finnigan SpectraSYSTEM.
Components: Automatic autosampler, AS 1000.
ChromQuest work support.
ION CHROMATOGRAPH WITH AMPEROMETRIC DETECTOR AND CONDUCTIVITY DETECTOR.
Dionex ICS3000 Liquid Chromatograph.
Amperometric pulse detector.
Conductivity detector.
Applications
- GAS CHROMATOGRAPHY
- COUPLED TO MASS SPECTROMETRY:
Analysis of drugs, pesticides, pharmaceutical products, proteins, polypeptides, steroids, fatty acid methyl esters, etc.
GC-MS, P&T: This technique allows the analysis of volatile organic compounds (trihalomethanes, chlorobenzenes, BTEX, etc.).
- COUPLED TO MASS SPECTROMETRY:
LIQUID CHROMATOGRAPHY
WITH FLUORESCENCE DETECTOR (HPLC-FL).
Quantify low concentrations of fluorescent compounds.
Polycyclic aromatic hydrocarbons in environmental samples (soils, sediments, waters…) and foods.
Compounds that can migrate from packaging to foods.
Drugs, vitamins…any compound that presents chemiluminescent properties.
ION CHROMATOGRAPH WITH AMPEROMETRIC DETECTOR AND CONDUCTIVITY DETECTOR.
Amperometric detector: monosaccharides, polysaccharides, aminoglycosides, antibiotics, sialic acids, etc.
Conductivity detector: analysis of anions in drinking water, mineral waters, beverages, etc.
Sample requirements
Samples must be presented correctly identified and numbered in a correlative manner.
Contact the laboratory to establish the conditions for the sample analysis.