Nuclear Magnetic Resonance

Nuclear Magnetic Resonance Spectroscopy is based on the observation of transitions between energy levels of the nuclei of certain atoms. The separation of said energy levels occurs when the sample is subjected to a very intense magnetic field. The observation of the transitions is carried out using electromagnetic radiation that is in the radio frequency range. The equipment installed in the Service is capable of performing any type of experiment collected in the extensive library of microprograms of the Bruker company or any variation of them that the user desires, provided that the pertinent modification is indicated and, very especially, the necessary phase programming. A wide range of mono and bidimensional spectra can be performed as standard experiments.

High-resolution 1H spectra can be routinely performed with all the usual variations in this technique: double resonance, solvent suppression, relaxation time determination, Overhauser nuclear effect (nOe) measurements, etc. In 13C spectra, spectra with total decoupling are considered routine experiments. DEPT experiments with an angle of 45, 90 or 135 degrees, etc. 31P and 19F experiments can also be routinely performed. The acquisition of spectra of the rest of the nuclei that can be observed with any of the available probes will be considered, in principle, as non-routine experiments, and it is necessary to contact the Service in advance to see the availability of the time necessary to make the adjustments required by said experiments.

Two-dimensional experiments: The following experiments are optimized: proton homonuclear correlation (COSY 45 or 90) Two-dimensional J-resolved spectra, NOESY, ROESY, TOCSY. H/C correlation spectra. The standard experiment performs the correlation to a bond. If a different correlation is desired, the user must indicate the type of experiment and the adjustment constant to be focused on.